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Biochemistry

Dive into the chemical processes and substances occurring within living organisms, focusing on biomolecular analysis.

Protein Estimation

Quantitative analysis of proteins using colorimetric methods like Bradford or Lowry assays.

Separation of proteins based on their molecular weight using polyacrylamide gel electrophoresis.

Separation of plant pigments, amino acids, or lipids using Paper Chromatography or Thin Layer Chromatography (TLC).

Determining the Michaelis-Menten constant (Km) and maximum velocity (Vmax) of an enzyme like Catalase.

Desalting and purifying protein samples using a semi-permeable dialysis membrane.

Qualitative analysis of sugars using Benedict's, Barfoed's, and iodine tests.

Extraction and qualitative identification of lipids from biological samples.

Identification of specific proteins from a complex mixture using antibody labeling.