Lab Experiment

BiotechNotesHub Experiments

Molecular Biology

• Genomic & Plasmid DNA Isolation
• Agarose Gel Electrophoresis
• Polymerase Chain Reaction (PCR)
• Restriction Enzyme Digestion
• DNA Ligation & Transformation

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Microbiology

• Preparation of Culture Media
• Sterilization Techniques
• Pure Culture Techniques
• Gram Staining & Growth Curves
• Biochemical Tests (IMViC)

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Biochemistry

• Protein Estimation (Bradford/Lowry)
• SDS-PAGE
• Chromatography Techniques
• Enzyme Kinetics Analysis
• Dialysis & Desalting

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Immunology

• ABO and Rh Blood Grouping
• ELISA & Radial Immunodiffusion
• Ouchterlony Double Diffusion
• Widal & VDRL Tests
• Immunoelectrophoresis

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Plant Biotechnology

• Preparation of MS Medium
• Surface Sterilization of Explants
• Callus Induction
• Micropropagation
• Protoplast Isolation

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Bioprocess Engineering

• Batch Fermentation (Ethanol)
• Citric Acid Production
• Immobilization of Cells/Enzymes
• Cell Disruption Techniques
• Downstream Processing

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Bioinformatics

• Sequence Retrieval (NCBI, EMBL)
• BLAST Analysis
• Multiple Sequence Alignment
• Phylogenetic Tree Construction
• Protein Structure Visualization

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