Bioinformatics (Dry Lab)
Master computational biology by exploring genetic databases, sequence alignment, and 3D protein structure visualization.
Sequence Retrieval
Fetching and managing DNA/Protein sequences from primary databases like NCBI, EMBL, and DDBJ.
Read ProtocolBLAST Analysis
Using the Basic Local Alignment Search Tool (BLASTn/BLASTp) to find regions of local similarity between sequences.
Read ProtocolMultiple Sequence Alignment
Using Clustal Omega or MUSCLE to align three or more biological sequences for evolutionary analysis.
Read ProtocolPhylogenetic Trees
Constructing and interpreting evolutionary trees using MEGA software via Maximum Likelihood methods.
Read Protocol3D Protein Visualization
Retrieving PDB files and viewing complex 3D macromolecular structures using PyMOL or RasMol.
Read ProtocolPrimer Design
Designing optimal forward and reverse primers for PCR applications utilizing Primer3 software.
Read ProtocolORF Finding
Locating Open Reading Frames (ORFs) within a raw genomic DNA sequence to identify potential protein-coding genes.
Read ProtocolHomology Modeling
Predicting the 3D structure of a target protein based on its alignment to a known template using SWISS-MODEL.
Read Protocol
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