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Plant Biotechnology

Techniques for growing plant cells, tissues, and organs under sterile and controlled in vitro laboratory conditions.

MS Medium Preparation

Formulating the standard Murashige and Skoog nutrient media required for plant tissue culture.

Preparing seeds, leaves, or nodal segments for culture by eradicating surface microbes safely.

Initiating an unorganized, dividing mass of plant cells (callus) from a specific plant explant.

Clonal mass propagation of plants using shoot tip, nodal culture, or axillary bud proliferation.

Enzymatic removal of rigid plant cell walls to isolate viable, naked protoplasts for fusion experiments.

Using the chemical indicator Tetrazolium chloride to evaluate the respiratory activity of plant embryos.

Using Agrobacterium tumefaciens as a vector to integrate foreign genes into the plant genome.

Encapsulating somatic embryos or shoot buds in sodium alginate beads for artificial seed production.