Polymerase Chain Reaction (PCR) Quiz – Part 5 (Advanced Variants & Troubleshooting)

Attempt all 10 questions. Click Submit to check your score and correct answers.

41. Which PCR variant is BEST suited for quantifying mRNA expression levels?

a) Nested PCR
b) RT-qPCR
c) Digital PCR
d) Hot-start PCR

42. A PCR reaction shows multiple nonspecific bands. Which adjustment is MOST helpful?

a) Lowering annealing temperature
b) Increasing primer concentration
c) Increasing annealing temperature
d) Decreasing Mg²⁺ concentration

43. Which PCR method is MOST suitable for detecting rare alleles in a heterogeneous population?

a) Touchdown PCR
b) Digital PCR
c) Multiplex PCR
d) Gradient PCR

44. In case of primer-dimer formation, which modification is MOST effective?

a) Reduce template concentration
b) Increase extension time
c) Redesign primers
d) Add more Mg²⁺ ions

45. Which PCR variant is MOST useful for detecting viral load in patients?

a) Nested PCR
b) RT-qPCR
c) Digital PCR
d) Hot-start PCR

46. Which of the following is a common cause of PCR failure?

a) Incorrect primer design
b) Absence of Mg²⁺ ions
c) Poor template quality
d) All of the above

47. Which modification in PCR helps to reduce contamination issues?

a) Hot-start PCR
b) Nested PCR
c) Use of uracil-DNA glycosylase (UDG)
d) Touchdown PCR

48. Which PCR technique is MOST used for single-cell analysis?

a) Digital PCR
b) Multiplex PCR
c) RT-PCR
d) Gradient PCR

49. Which step would you modify to increase yield without affecting specificity?

a) Increase cycle number
b) Decrease extension time
c) Reduce primer length
d) Increase annealing temperature

50. Which advanced PCR technique allows absolute quantification without reference standards?

a) qPCR
b) Digital PCR
c) RT-PCR
d) Hot-start PCR

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