🧫 Colony Picker: Blue/White Screen
You just transformed E. coli with your cloned plasmid and plated them on LB Agar with Ampicillin and X-gal. It is time to harvest the successful clones before the incubator timer runs out!
⚪ WHITE Colonies: Tap these instantly! The target gene successfully inserted and disrupted the lacZ gene. These are your Recombinants!
🔵 BLUE Colonies: Avoid these! The plasmid closed up empty. The lacZ gene is still intact and is digesting the X-gal to turn blue.
🦠 GREEN Fuzz: Contamination! Do not touch the mold!
🚨 EXAM TRAP REMINDER: An intact lacZ gene produces Beta-galactosidase, which cleaves X-gal into a BLUE product. Therefore, Blue = Empty Plasmid. White = Successful Insert!
Clones
0
Purity
100%
Time
30
Awaiting incubator...
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