๐งช ELISA Express V2: False Positive Panic
The Immunology Lab is overflowing with patient samples. Build the Sandwich ELISA, but whatever you do... DO NOT FORGET TO WASH!
- ๐งฒ 1. Capture Ab (Coat the well)
- ๐ 2. WASH PLATE!
- ๐ฆ 3. Antigen (Add patient sample)
- ๐ 4. WASH PLATE!
- ๐ 5. Enzyme-Ab (Complete the sandwich)
- ๐ 6. WASH PLATE!
- ๐ง 7. Substrate (Develop the color!)
- ๐จ THE TWIST: If you skip a wash step, unbound proteins remain in the well. When you add substrate, the whole well turns brown (False Positive!) and your assay is ruined!
Wells Read
0
Combo
x1
Time
30
Automated Washer Offline. Press Start.
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