[attachment_0](attachment)
🧬 PCR Master: Thermal Cycler Dash
Take control of the Thermal Cycler! You must manually shift the temperatures in the exact correct order to exponentially amplify your target DNA.
🔥 1. Denature (95°C): Break the hydrogen bonds to separate the double helix into single strands.
❄️ 2. Anneal (55°C): Cool it down so the forward and reverse primers can bind to the target sequences.
⏩ 3. Extend (72°C): The optimal temperature for Taq Polymerase to synthesize the new complementary strands!
🚨 EXAM TRAP: You cannot run PCR without a Master Mix! You MUST add Taq Polymerase, dNTPs, and the critical Mg²⁺ cofactor before heating the tube!
DNA Copies
1
Cycle
0
Time
30
Thermal Cycler Offline. Press Start.
No comments:
Post a Comment