๐งฌ Operon Overdrive: The Lac Switch
Master the most heavily tested concept in molecular biology! Analyze the cellular environment and the genotype, then instantly predict the expression level of the lac operon.
- ๐ด OFF: Repressor is bound. (Happens when Lactose is ABSENT).
- ๐ก BASAL (Low): Repressor is removed, but no CAP-cAMP. (Happens when Lactose is PRESENT, but Glucose is also PRESENT).
- ๐ข HIGH (Max): Repressor removed AND CAP-cAMP bound! (Happens when Lactose is PRESENT, and Glucose is ABSENT).
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๐จ EXAM TRAP MUTANTS:
• lacI(s) = Super-repressor. It NEVER falls off. Transcription is ALWAYS OFF.
• lacO(c) = Operator mutant. Repressor CANNOT bind. It will transcribe even without Lactose!
Score
0
Combo
x1
Time
30
Glc: HIGH
Lac: LOW
WT
System Ready. Awaiting Initialization.
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